WebThe BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine … WebFluorochromes have unique and characteristic spectra for absorption (excitation) and emission. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. A tandem dye consists of a donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two.

Fluorophore Families - BioLegend

WebAt BioLegend, we've spent nearly two decades crafting new fluorophores for flow cytometry. With each new option, researchers and colleagues have come to trust the performance of our products, as evidenced by this … WebCD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes ... lithium chloride + sodium

BioLegend

WebBiolegend: Antibodies, buffers and other flow cytometry reagents. BU/BMC users are eligible for up to 25-35% discount, ... Lists most recent BD dyes including Brilliant Violet and Brilliant UV Sirigen fluorochromes: Fluorochrome chart: Lists most common fluorochromes, their excitation and emission and optimal filters sorted by brightness ... Web38 rows · BioLegend provides a convenient Fluorophore Brightness Index as a tool for multicolor flow cytometry. The Fluorophore Brightness Index Score is a relative … WebThere are percentages of a population, fluorescence intensity measurements, sample averages, data normalization, and more. Many of these common calculations are useful, but surrounded by misconceptions. This primer will help you decide which calculation to use, when to use it, and how to interpret the results. 1. Staining Index. impulse and reaction turbine examples