Web1 hour ago · This study emphasizes that functional assays in relevant tissues can refine likely causal genes from GWASs and implicates new regulators of chondrocyte maturation. ... We identified 145 genes that alter chondrocyte proliferation and maturation at early and/or late time points in culture, with 90% of genes validating in secondary screening. ... WebProliferation Assay of Chondrocytes. Chondrocytes were seeded into 24-well plates and cultured to achieve 70–80% confluence for use in proliferation assay. THP-1 cells (1.6 × 10 4 cells/cm 2) were seeded in the upper layer of a 24-well transwell chamber (pore size: 0.4 μm, Corning) whereas PBS buffer was added to the lower chamber ...
Mechanical stimulation promotes the proliferation and …
WebThree methods are presented in this unit for culturing chondrocytes, and two assays are described that characterize proteoglycan synthesis, a key measure of chondrocyte function.Chondrocytes constitute the sole cell type found within cartilage, and control the formation and composition of cartilage. MeSH terms Animals WebJan 15, 2024 · Chondrocytes, the sole kind of cell in cartilage tissue, are essential to preserving the homeostasis of cartilage tissue [ 18 ]. However, the homeostasis of cartilage tissue is readily disturbed because of the unique organizational features of cartilage tissue—no blood vessels, nerves or lymphatics [ 19 ]. slr for plant diseases recognization
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WebApr 12, 2024 · Acrylamide (ACR) is formed during heat treatment of foodstuffs and ACR may serve as a probable malignant neoplastic disease agent in all organs and tissues of the human body. However, it is unknown if ACR is associated with ankylosing spondylitis (AS) pathogenesis. Cell viability and proliferation were determined using CCK‑8 assay and … WebSep 25, 2014 · One of the signaling pathways regulating chondrocyte proliferation and differentiation is mediated through G proteins. ... Then the cell viability was determined by the MTT assay in triplicate. Absorbance was measured by using a microplate reader at the wavelength of 570 nm with a reference wavelength of 630 nm. WebJan 25, 2024 · Effects of deer TGF-β1 on chondrocyte proliferation and differentiation related genes. We further investigated the expression of genes critical for chondrocyte proliferation and differentiation by … slr food supplier