Tk minimal promoter
WebPromoter: Mini-TK promoter Replicon: pUC ori Terminator: SV40 poly (A) signal Plasmid classification: lactation serial plasmid; lactating reporter plasmid; luciferase plasmid Plasmid size: 5539bp Plasmid tagging: Luc Prokaryotic resistance: Amp (100 g/ml) Cloning strains: E. coli DH5 and E. Culture conditions: 37 C, LB, aerobic. Webin cell extracts were determined after 60 hours of growth. HR5 clones with a CMV minimal promoter in front of the reporter gene are referred to as HR5-C, and those with the TK minimal promoter are referred to as HR5-T (11). L designates the luciferase and Z the ,B-galactosidase reporter gene. The data show that the
Tk minimal promoter
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WebSequence, structure and promoter characterization of the human thymidine kinase gene. The 12.9-kb human thymidine kinase gene (tk) has been sequenced in its entirety along … Web22 dic 2009 · This library of potential enhancers was introduced into a retroviral vector and screened in multiple cell lines for the ability to activate GFP transcription from a minimal …
WebThe BPV-1 E2 protein does not efficiently activate a minimal promoter containing E2 DNAbinding sites and the tk TATAbox alone. C33 ... at position -74 in the tk promoter and the XbaI site at -109. WebFOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash …
Web8 apr 2024 · The pRL-TK vector containing the Renilla luciferase gene under HSV-TK minimal promoter (Promega, Madison, WI, USA) were co-transfected with each construct and served as an internal control for ... WebEl VWF es una glicoproteina adhesiva, sintetizada en las CE y megacariocitos. Se sintetiza en forma monomerica, luego se organiza en dimeros y se multimeriza.
WebTranscription preinitiation complex, represented by the central cluster of proteins, causes RNA polymerase to bind to target DNA site. The PIC is able to bind both the promoter sequence near the gene to be …
Web19 dic 2005 · The thymidine kinase (Tk) minimal promoter was prepared by excision from the vector pBLCAT2 (GenBank X64410, 435–603) using BglII and BamHI and inserting the fragment into the BamHI site of pEGFP. mp3 with app storeWebThe 12.9-kb human thymidine kinase gene (tk) has been sequenced in its entirety along with flanking regions. Consistent with the previously sequenced chicken tk sequence, the human tk is composed of seven exons. The intron sizes differ substantially, and are responsible for the four-fold greater siz … mp3 with app store supportWeb1 apr 1997 · Systematic 3′ deletions of this XhoI–KpnI fragment yielded pHHXE, pHHXB, pHHXN, and pHHXX, respectively (see Fig. 3 for details). For construction of ptk−, the TIE2 promoter of pHH− was replaced with the herpes simplex virus 1 thymidine kinase gene (tk) minimal promoter (nucleotides −116 to +68) from PMC1POLA (Stratagene). ptkXK and ... mp3 with am fm radioWebHos TK promotion har vi de bedste boksere i landet. De er med i events og stævner over hele landet. Så er du til boksekampe - Book billet nu mp3 with dapWeb24 nov 2010 · The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are … mp3 with auxWebDear researcher, minimal promoter refers to a minimal sequence of a native promoter (mostly core promoter) that could express a downstream gene. So, that not mean … mp3 with batteriesWeb15 giu 2006 · A plasmid containing five GAL4-binding sites upstream of a TK minimal promoter and the firefly luciferase gene was used as a reporter. COS-7 cells were grown in the absence or presence of 9-cis retinoic acid (1 μM) as indicated. mp3 with bluetooth reviews